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. 2005 Oct 20;33(18):6070–6080. doi: 10.1093/nar/gki917

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© The Author 2005. Published by Oxford University Press. All rights reserved

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The effect of increasing TMPyP4 and telomestatin concentrations on the conversion of 59mer duplex DNA (59WT) to alternative secondary structures. (A) Nucleotide sequence of 59WT. (B) Structures of TMPyP4 and telomestatin. (C) Autoradiogram of DNA breathing assay. Duplex DNA was titrated with TMPyP4 or telomestatin in 100 mM KCl/TE buffer at 37 or 42°C. Two bands corresponding to Watson–Crick duplex (DS) and single-stranded DNA (S) were identified, along with an isomeric form. (D) Graphical representation of data described in (C).