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. 2005 Oct 20;33(18):6070–6080. doi: 10.1093/nar/gki917

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© The Author 2005. Published by Oxford University Press. All rights reserved

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DNA polymerase stop assay to determine the ability of the VEGF promoter to form G-quadruplex structures. (A and B) The wild-type (WT) and mutant (MT) template sequences (shown below the gels) with increasing concentrations of K⁺ (0–100 mM). Arrows indicate the positions of the full-length product (F) of DNA synthesis, the G-quadruplex pause site (S), and the free primer (P). Lanes A, G, T and C represent dideoxy sequencing reactions with the same template as a size marker for the precise arrest sites.