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. 2002 Jun 27;99(15):9960–9965. doi: 10.1073/pnas.132254199

Figure 1.

Figure 1

Strong but incomplete tolerance to glycosylated CII in humanized mice. Recall in vitro response of lymph node cells from DR4 and huCII/DR4 mice immunized 10 days earlier with human CII (huCII). Cells were restimulated with huCII and the following CII-peptides: nonglycosylated (K264/270), glycosylated at hydroxylysine 264 (Gal264), glycosylated at hydroxylysine 270 (Gal270), or glycosylated at both hydroxylysine residues (Gal264/270) with β-d-galactopyranosyl residues. Ten animals (5 males and 5 females) of each mouse line were investigated. As a positive control, the recall response to mycobacteria antigen PPD (purified protein derivate, present in complete Freund's adjuvant) was measured. The response in huCII/DR4 mice was significantly reduced against all CII antigens (P < 0.05) as compared with DR4 mice, except for Gal264/270-specific IFN-γ production. *, Significant responses above background levels for huCII/DR4 mice. *, P < 0.05, Mann–Whitney U test; bars represent mean ± SEM. Antigen-specific proliferation and IFN-γ production: Δcpm and ΔU/ml respectively (response with antigen—response in the absence of antigen).