Figure 4.

Specific recognition of CII-glycopeptides by human T cells. The two-color flow-cytometry of in vitro-stimulated T cells from an RA-patient shows fluorescence intensities for surface-binding of a FITC-labeled anti-CD4 antibody on the x axis whereas the y axis represents signal intensities for intracellular staining with a phycoerythrin-labeled anti-IL-2 antibody. PBMCs were cultured overnight without antigen, with tetanus toxoid, the K264/270-peptide, or the Gal264-peptide. The figures in the upper right quadrant of the different panels represent the percentage of double-positive cells.