Table 1.

DR4-restricted T cell hybridoma responses to the CII259–273 epitope and human CII

Semiquantitative scoring of T cell hybridoma response following stimulation with glycosylated or nonglycosylated collagen peptides (see Fig. 1). Shaded boxes highlight specific responses for the different groups of clones. 

^*Sensitivity of the T cell hybridomas was determined by the amount of peptide required for a CTLL response >1000 CPM above background; 0, no response; 1 = 50 μg/ml; 2 = 10 μg/ml; 3 = 2 μg/ml; 4 = 0.4 μg/ml; 5 = 0.08 μg/ml; 6 = 0.016 μg/ml. 

^† Sensitivity of the T cell hybridomas to CII protein was determined as for the CII-peptides, but number indicates a 5 times higher concentration of antigen, i.e., 1 = 250 μg/ml; 2 = 50 μg/ml; 3 = 10 μg/ml; 4 = 2 μg/ml; 5 = 0.4 μg/ml; 6 = 0.08 μg/ml.