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Replicative life span of parental and derived oral keratinocytes. Growth characteristics of OKF6, OKF6-D1, OKF6-dnp53, OKF6-D1/dnp53, OKF6-D1/dnp53/EGFR, and OKF6-D1/dnp53/EGFR plus EGF. The replicative life span was assessed by calculating the PDs of each cell line. Immortalization was assessed if cells grew at least three times beyond the life span of the parental cells.
