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. 2005 Oct 20;102(43):15599–15604. doi: 10.1073/pnas.0409730102

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Copyright © 2005, The National Academy of Sciences

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Fig. 3. — Telomerase activity and telomere length in parental and derived oral keratinocytes. (a) Cellular extracts (100 ng) of OKF6-hTERT, OKF6-D1, OKF6-dnp53, OKF6-D1/dnp53, OKF6-D1/dnp53/EGFR, as well as OKM1-D1/dnp53, OKM1-D1/dnp53/EGFR, and OKM1-D1/dnp53/EGFR/c-myc cells were assayed for telomerase activity using the PCR-based TRAP assay. Heat-treated (heat) samples served as negative control, and OKF6-hTERT served as a positive control. IC is an internal PCR control. (b) Telomere length for preimmortal OKF6-D1/dnp53 (100 PD), immortal OKF6-D1/dnp53 (200 PD), OKF6-D1/dnp53/EGFR, OKF6-D1/dnp53/EGFR/empty, and OKF6-D1/dnp53/EGFR/c-myc cells was analyzed by hybridization of genomic DNA with a specific oligonucleotide probe. Two different clones of OKF6-D1/dnp53/hTERT cells served as positive control.