Table 1. Colony formation rate in soft agar analysis.
Colonies in soft agar
|
||
---|---|---|
Cell type | 104 cells | 105 cells |
OKF6-D1/dnp53 (plus EGF) | 0 (2) | 2 (2) |
OKM1-D1/dnp53 (plus EGF) | 0 (0) | 0 (2) |
OKF6-D1/dnp53/empty | 1 | 1 |
OKM1-D1/dnp53/empty | 1 | 2 |
OKF6-D1/dnp53/EGFR | 50 | 163 |
OKM1-D1/dnp53/EGFR | 55 | 135 |
OKF6-D1/dnp53/EGFR/c-myc | 59 | 162 |
OKM1-D1/dnp53/EGFR/c-myc | 53 | 149 |
TE-12 | 61 | 153 |
Results from two individual clones derived from each set of experiments gave identical results. Experiments were done in triplicate. Colony formation was scored after 3 weeks.