Table 1. PCR and sequencing primers, in the 5′ to 3′ direction, for the seven loci used in this study.
Gene | Forward | Reverse |
---|---|---|
Signal peptidase | TTGCCATTTTATGCTGAGCTGAAT | TTTTGGAGCTTCAAGGCTTTATTT |
CCGCTTGTATAGTAGCTGCCATTG | CAACACACAATTAGAACCGTTTTCG | |
Unknown P1439 | GCCTCAAAGAGTGAAAGAGGGTTT | ATCCAGAGGTAGCAAACGGCTTAT |
TCACAGTCCTTTAGCCTGATACTACC | ||
Clathrin light chain | GCCGTGAGGGTCTTTGGTTAAG | CGGTATATAGTATGGGCCTTGGTTC |
TCCTCCATGATAAATGACATGCAA | CAGGAATAGTGCTATCGCGTTCAT | |
PTTGIIP | TACTTGATCACAGTTCACGCCAAA | CCTATACAGCGCTGATTGCTGATG |
Phospholipase A21-precursor | CAGCACGTACTGTGTATCGAAAGAA | AAAAGCAATTTGGAACAAAGTATGAA |
TCATCTGTCGCAGGTACGTATGAA | ACTTGTATCTGGCGAAGCATTCAG | |
TTTTGTGCGCGCTAAAACTATCTT | ||
CTGGTAGCTGTGACCGTGCTAACT | ||
Unknown P1500 | ATCCCGCGTTCTCCTACTATTTTT | AATTTTATGATCGCTTCCTGCAAT |
TCATTCGTCGTGTGGCTGTTACTA | GCACAAGTATTAACCACACCGTCA | |
GCGCCTTGGTTTCTACTGTTTTGT | ||
Glutathione S-transferase | GGTGTTGCCTTGTGTTCACTTTT | GGGATGCAAAACTAATAATTACCAAA |
TGGTCAAATTCGTCAACTTTACGTTC | TTTCTTAGATTTGGCTGGAAAGAC |
The first primer pair for each gene was designed to the 5′ and 3′ UTR; subsequent primers listed below these spanning primers are internal primers. All PCRs were carried out by using the 5′ and 3′ UTR primers and sequencing was performed using all primers listed.