Fig. 2.

Formation of covalent compounds between APF-1 and lysozyme in an ATP-dependent reaction. Tracks 1–5, compound formation with ¹²⁵I-APF-1; track 1, without ATP; track 2, with ATP; tracks 3–5, with ATP and 5, 10, or 25 mg of unlabeled lysozyme, respectively; and tracks 6 and 7, compound formation with ¹²⁵I-lysozyme. Conditions were as detailed in Methods of ref. 7, except that 5 μgof ¹²⁵I-lysozyme (40,000 cpm) and 3 μgof unlabeled APF-1 were used. Track 6, ATP omitted; track 7, with 2 mM ATP. Contamination in ¹²⁵I-labeled lysozyme is indicated. [Reproduced with permission from ref. 7 (Copyright 1980).]