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Proposed sequence of events in ATP-dependent protein breakdown (see the text). 1, APF-1-protein amide synthetase (acting on lysine ε-NH2 groups). 2, Amidase that allows correction when n = 1 or 2. 3, Peptidases that act strongly on (APF-1)n derivatives, when n > 1 or 2. 4, Amidase for APF-1-X; X is lysine or a small peptide. [Reproduced with permission from ref. 7 (Copyright 1980).]
