Table 2.
Sμ deletions in WT and AID−/− hybridomas
| Probe/digest
|
||||
|---|---|---|---|---|
| JH/S or E | Iμ/X | Sμ/X | Cμ/B+E | |
| WT IgM+ hybridomas with Sμ rearranged: 15/117 (12.8%) | ||||
| W-1 | R/R | G/R | G/R | G/R |
| W-2 | −/R | R/R | −/R | R/R |
| W-3* | −/R | −/− | −/R | −/R |
| W-4 | R/R | G/R | G/R | G/R |
| W-5 | R/R | R/R | R/R | R/R |
| W-6 | R/R | R/R | R/R | R/R |
| W-7 | R/R | G/R | G/R | G/R |
| W-8 | R/R | G/R | G/R | G/R |
| W-9 | −/R | nd | −/R | −/R |
| W-10 | R/R | nd | G/R | G/R |
| W-11 | R/R | nd | R/R | R/R |
| W-12 | R/R | nd | G/R | G/R |
| W-13 | R/R | nd | −/R | −/R |
| W-14 | R/R | nd | −/− | −/R |
| W-15 | R/R | nd | −/R | −/R |
| WT controls | ||||
| C-1 (IgG+) | R/R | −/− | −/− | −/− |
| C-2 (IgM+) | R/R | nd | G | G |
| AID−/− IgM+ hybridomas with Sμ rearranged: 5/214 (2.3%) | ||||
| A-1† | −/R | G | G/R | G/R |
| A-2 | R/R | G/R | G/R | G/R |
| A-3 | R/R | G/R | G/R | G/R |
| A-4† | −/R | G | G/R | G/R |
| A-5 | −/R | G/R | G/R | G/R |
nd, not done; G, germ line; R, rearranged; −, undetectable or comigrating band; B, BamH1; E, EcoR1; H, HindIII; S, Sac1; X, Xba1.
*
A rearranged Iμ band is detectable by using a BamH1 + EcoR1 double digest.
†
Sμ rearrangements appear to be the results of aberrant V(D)J joining.