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. 2002 Jul 15;99(15):9996–10001. doi: 10.1073/pnas.142309999

Figure 3.

Figure 3

Matrix accumulation in chondrocyte-seeded peptide hydrogel. (A) Total GAG accumulation in cell-seeded peptide hydrogel cultured in FBS and ITS/FBS medium and in cell-seeded agarose. (B) Toluidine blue staining of chondrocyte-seeded peptide hydrogel cultured in 10% FBS, day 15. (C) Immunohistochemical staining for type II collagen in cell-seeded peptide hydrogel cultured in 10% FBS, day 15. Image width for B and C = 175 μm. (D) SDS/PAGE of collagens extracted from day 35 samples of chondrocyte-seeded peptide hydrogel cultured in 1% ITS with 0.2% FBS. Standards: Chick cartilage for collagen II and XI banding pattern. Mouse skin identifies collagen I α-helix 2, indicative of collagen expression of a dedifferentiated, fibroblastic phenotype.