Fig. 5.

Converting Mma Lrp into a potent transcriptional activator. (A) Comparison of activation levels elicited by 250, 500, or 1,000 nM Mja Ptr2 (lanes 2–4, respectively), wild-type Mma Lrp (lanes 5–7), mutant 1 (lanes 8–10), mutant 2 (lanes 11–13), and mutant 3 (lanes 14–16), in conjunction with Mja TBP, Mth TFB, and Mth RNAP. Single rounds of transcription were carried out at 37°C in the presence of 250 mM NaCl (and in the absence of thermoprotectants). (B) Levels of activation; data from A.(C) Transcriptional response to wild-type Mma Lrp by the three methanococcal TBPs. Single rounds of transcription were carried out as in A. (D) Transcriptional response to Mma Lrp mutant 1 by the three methanococcal TBPs.