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. 2002 Jul 3;99(15):10042–10047. doi: 10.1073/pnas.142005099

Figure 2.

Figure 2

Increased bleeding time with decreased levels of VWF and Factor VIII occurs with ST3Gal-IV deficiency but not with ST6Gal-I deficiency. (A) Bleeding times were analyzed among 6- to 12-wk-old littermates of the indicated genotypes. Results are from over 30 mice of each ST3Gal-IV genotype and 11 of each ST6Gal-I genotype are expressed as means ± SEM. Significantly increased bleeding times for ST3Gal-IVΔ heterozygotes and homozygotes were observed (P < 0.05 and P < 0.001, respectively). (B) VWF levels in plasma were measured from over 30 mice of each genotype and plotted as a percentage of the mean value obtained from ST3Gal-IV wild-type littermates. A significant reduction in plasma VWF was observed in both heterozygous and homozygous ST3Gal-IV-deficient mice to a mean of 57 and 50%, respectively (P < 0.001). No decrease in plasma VWF levels was observed in mice lacking ST6Gal-I. (C) Factor VIII (F VIII) levels were also reduced in mice analyzed in B (P < 0.001). ST6Gal-I deficiency had no effect on F VIII levels in circulation (not shown). (D) Levels of blood coagulation factors were analyzed from 40 mice of indicated genotypes. No alterations of hemostatic significance were observed.