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. 2002 Jul 3;99(15):10042–10047. doi: 10.1073/pnas.142005099

Figure 4.

Figure 4

Decreased VWF half-life and platelet homeostasis corrected by blocking ASGPR. (A) Plasma fractions from mice of indicated genotypes were biotinylated and injected into wild-type mice in the presence or absence of 10 mg of pyrogen-free asialofetuin. Biotinylated VWF levels in blood were analyzed at the indicated times. Data are derived from three mice per treatment group and are representative of two separate experiments. In additional experiments that included native fetuin, only asialofetuin was able to block ASGP clearance and increase half-life (data not shown). (B) RCA lectin binding to platelets was increased among blood samples from mice homozygous for the ST3Gal-IV mutation as detected by flow cytometric analysis. A slight increase in RCA-I binding to platelets is seen in heterozygous samples. Data are representative of three separate experiments. (C) Mice homozygous for the ST3Gal-IV gene deletion were injected with asialofetuin or fetuin at 0, 4, and 8 h. Platelet levels were analyzed by flow cytometry of whole blood at the timepoints indicated. Data are the means ± SEM from 10 mice per group.