Figure 4.

Proliferation and viral expression. (A) Two days after BrdUrd injection, PBMCs from noninfected (no. 1097), asymptomatic (no. 8), and lymphocytic (nos. 2668 and 2658) sheep were isolated and cultivated during 18 h. The cells then were fixed and incubated with anti-p24 antibody 4′G9, which recognizes the viral capsid protein, and with a PE-conjugated secondary antibody. Finally, cells were stained with anti-BrdUrd FITC with DNase and analyzed by flow cytometry. A representative experiment (out of three) is represented by dot plots (10,000 selected events). Numbers represent the percentages of positively stained cells in each quadrant. (B) Schematic representation of BrdUrd incorporation in vivo (hypothetical) and p24 labeling after short-term culture (ex vivo). Hatched areas represent the uninfected cell; black squares and full circles indicate BrdUrd and p24 markers, respectively. Crosses indicate that cells were undetectable by FACS.