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PLA2 activity of rSla. PLA2 activity was assayed with a commercially available kit that measures the hydrolysis of phospholipids at the sn-2 position. The assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine as a chromogenic substrate. Bee venom PLA2 was used as positive control (data not shown) and rSpeK was used as negative control.
