Table 1.
Toxin | Pyrogenicity* (Avg. change, °C) | Models of lethality (No. dead per no. total animals)
|
|
---|---|---|---|
Endotoxin† enhancement | Miniosmotic‡ pump | ||
rSpeK | +1.3 | 3/3 | 2/3 |
rSSA | +1.4 | 3/3 | 3/3 |
rTSST-1 | +0.9 | 3/3 | 3/3 |
PBS | −0.1 | 0/3 | ND§ |
Animals were injected intravenously with 5 μg/kg of recombinant protein at 0 h, and temperatures were recorded at 4 h. Three rabbits were used for all assays with the exception of rTSST-1, in which six were used.
Mortality was recorded for a 48-h period after 10 μg/kg endotoxin injection.
Mortality was recorded for 15 days after subcutaneous implantation of pump.
Not determined.