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. Author manuscript; available in PMC: 2005 Oct 26.
Published in final edited form as: J Biol Chem. 2005 May 24;280(29):26796–26804. doi: 10.1074/jbc.M503805200

Figure 2. Characterization of detergent-resistant membranes prepared using 1% Brij 98.

Figure 2

CHO cells were solubilized with 1% Brij 98 and the extracts analyzed by sucrose density gradient centrifugation as described in Materials and Method. Gradients were fractionated and equal volumes of each fraction were separated by SDS polyacrylamide gel electrophoresis. Gels were transferred to Immobilon and subjected to Western blotting using the indicated antibody. Left gradient, sucrose solutions contained no Brij 98. Right gradient, sucrose solutions contained 0.5% Brij 98.