Supplemental Table 2.
Ethanolamine-containing Glycerophospholipids
|
PNS |
Detergent free |
Brij 98 |
Triton X 100 |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| m/z | Assignment | Average (nmol/mg protein) | S.D. | mol% Phospholipid | Average (nmol/mg protein) | S.D. | mol% Phospholipid | Average (nmol/mg protein) | S.D. | mol% Phospholipid | Average (nmol/mg protein) | S.D. | mol% Phospholipid |
| 658.5 | D14:1-16:1 | 1.43 | 0.67 | 0.56 | 2.38 | 1.70 | 0.26 | 0.74 | 0.06 | 0.02 | 1.52 | 0.01 | 0.06 |
| 672.5 | P16:0-16:1 | 2.10 | 1.04 | 0.82 | 3.41 | 0.59 | 0.37 | 4.35 | 0.25 | 0.13 | 5.29 | 0.26 | 0.21 |
| 686.5 | D14:0-18:2/D16:1-16:1 | 0.99 | 1.03 | 0.39 | 2.67 | 1.20 | 0.29 | 2.85 | 0.18 | 0.08 | 2.10 | 0.17 | 0.08 |
| 688.5 | D14:0-18:1/D16:0-16:1 | 0.88 | 0.62 | 0.34 | 3.02 | 1.52 | 0.32 | 2.65 | 0.04 | 0.08 | 2.02 | 0.10 | 0.08 |
| 698.5 | P16:0-18:2/P18:1-16:1 | 1.45 | 0.76 | 0.57 | 7.22 | 1.64 | 0.77 | 28.47 | 3.37 | 0.85 | 26.61 | 3.37 | 1.07 |
| 700.5 | P18:1-16:0/P16:0-18:1 | 4.83 | 1.87 | 1.90 | 28.63 | 7.52 | 3.07 | 109.88 | 4.86 | 3.27 | 88.59 | 11.82 | 3.57 |
| 702.5 | P18:0-16:0 | 3.41 | 1.84 | 1.34 | 0.55 | 0.67 | 0.06 | 0.00 | 1.88 | 2.66 | 0.08 | ||
| 712.5 | D16:1-18:2 | 1.35 | 0.93 | 0.53 | 2.01 | 0.52 | 0.22 | 3.60 | 0.18 | 0.11 | 2.64 | 0.37 | 0.11 |
| 714.5 | P16:0-19:1/D16:0-18:2/D16:1-18:1 | 1.76 | 0.89 | 0.69 | 8.27 | 1.45 | 0.89 | 19.28 | 0.80 | 0.57 | 11.28 | 1.62 | 0.45 |
| 716.5 | D16:0-18:1 | 3.05 | 1.24 | 1.20 | 11.18 | 2.87 | 1.20 | 31.66 | 3.40 | 0.94 | 31.68 | 7.54 | 1.28 |
| 720.5 | P16:1 -20:4 | 10.63 | 1.72 | 4.17 | 9.56 | 1.04 | 1.03 | 7.73 | 0.43 | 0.23 | 5.28 | 0.99 | 0.21 |
| 722.5 | P14:0-22:4/P16:0-20:4 | 4.04 | 1.78 | 1.59 | 29.04 | 14.00 | 3.12 | 143.02 | 4.31 | 4.25 | 47.95 | 17.01 | 1.93 |
| 724.5 | P16:0-20:3/P18:1-18:2 | 0.99 | 0.58 | 0.39 | 3.09 | 0.71 | 0.33 | 5.36 | 0.11 | 0.16 | 6.47 | 2.00 | 0.26 |
| 726.5 | P16:0-20: 2/P18:0-18:2/P18:1-18:1 | 2.45 | 1.01 | 0.96 | 13.14 | 4.91 | 1.41 | 45.40 | 1.94 | 1.35 | 20.78 | 2.08 | 0.84 |
| 728.5 | D17:1-18:1/P16:0-20:1/P18:0-18:1 | 3.75 | 1.62 | 1.47 | 22.08 | 2.60 | 2.37 | 70.35 | 1.41 | 2.09 | 57.18 | 9.54 | 2.31 |
| 730.5 | A18:0-18:1 | 1.29 | 0.68 | 0.51 | 3.34 | 2.20 | 0.36 | 4.31 | 1.91 | 0.13 | 1.33 | 0.38 | 0.05 |
| 732.5 | D14:1-22:6 | 2.08 | 0.74 | 0.82 | 5.40 | 3.73 | 0.58 | 2.05 | 0.45 | 0.06 | 1.04 | 0.37 | 0.04 |
| 734.5 | D16:2-20:4/D14:1-22:5 | 1.01 | 0.47 | 0.40 | 1.78 | 0.56 | 0.19 | 8.42 | 1.24 | 0.25 | _ | _ | 0.00 |
| 736.5 | D16:1-20:4 | 1.28 | 0.69 | 0.50 | 2.93 | 0.50 | 0.31 | 12.31 | 0.69 | 0.37 | 5.44 | 0.06 | 0.22 |
| 740.5 | D16:0-20:3/D18:1-18:2 | 2.94 | 1.10 | 1.15 | 4.15 | 0.28 | 0.45 | 12.22 | 1.39 | 0.36 | 8.55 | 1.00 | 0.34 |
| 742.5 | D18:0-18:2/D18:1-18:1 | 4.00 | 1.52 | 1.57 | 20.03 | 1.51 | 2.15 | 76.27 | 6.77 | 2.27 | 30.46 | 4.75 | 1.23 |
| 744.5 | D18:0-18:1 | 4.75 | 1.45 | 1.86 | 14.30 | 4.86 | 1.54 | 53.67 | 0.40 | 1.60 | 37.49 | 2.36 | 1.51 |
| 746.5 | D18:0-18:0/P16:0-22:6 | 2.22 | 1.07 | 0.87 | 6.59 | 5.10 | 0.71 | 48.02 | 4.14 | 1.43 | 10.88 | 0.63 | 0.44 |
| 748.5 | P18: 1-20:4/P16:0-22:5 | 8.95 | 1.37 | 3.52 | 16.92 | 11.91 | 1.82 | 92.06 | 13.63 | 2.74 | 23.29 | 2.96 | 0.94 |
| 750.5 | P18:1 -20:3/P18:0-20:4/P16:0-22:4 | 5.07 | 1.67 | 1.99 | 22.50 | 9.67 | 2.42 | 127.83 | 14.29 | 3.80 | 50.25 | 7.30 | 2.03 |
| 752.5 | P18:1 -20: 2/P18:0-20:3/P16:0-22:3 | 1.25 | 0.50 | 0.49 | 2.89 | 0.16 | 0.31 | 5.69 | 1.22 | 0.17 | 6.94 | 0.60 | 0.28 |
| 754.5 | P18:1-20:1/P18:1-20:2/P16:0-22:2 | 1.59 | 0.69 | 0.62 | 5.99 | 0.54 | 0.64 | 14.11 | 0.59 | 0.42 | 7.00 | 0.27 | 0.28 |
| 756.5 | P18:1 -20:0/P18:0-20:1/P16:0-22:1 | 1.67 | 0.81 | 0.65 | 7.44 | 0.60 | 0.80 | 17.55 | 1.44 | 0.52 | 13.42 | 0.28 | 0.54 |
| 758.5 | P18:0-20:0 | 2.43 | 1.05 | 0.95 | 9.09 | 2.93 | 0.98 | 10.19 | 0.20 | 0.30 | 10.17 | 0.44 | 0.41 |
| 760.5 | D16:1-22:6 | 1.72 | 0.26 | 0.67 | 3.59 | 1.08 | 0.39 | 11.64 | 2.97 | 0.35 | 5.89 | 1.41 | 0.24 |
| 762.5 | D16:0-22:6 | 1.38 | 0.71 | 0.54 | 2.27 | 0.28 | 0.24 | 7.70 | 1.12 | 0.23 | 3.09 | 0.47 | 0.12 |
| 764.5 | D18:1-20:4 | 2.80 | 0.81 | 1.10 | 5.20 | 0.72 | 0.56 | 20.46 | 3.85 | 0.61 | 10.12 | 0.59 | 0.41 |
| 766.5 | D16:0-22:4/D18:0-20:4 | 4.34 | 1.12 | 1.70 | 9.36 | 1.21 | 1.00 | 49.00 | 6.68 | 1.46 | 18.27 | 2.81 | 0.74 |
| 768.5 | D18:2-20:1/D18:1-20:27 | 1.44 | 0.81 | 0.57 | 3.61 | 1.29 | 0.39 | 7.65 | 0.51 | 0.23 | 3.96 | 0.25 | 0.16 |
| 770.5 | D18:1-20:1/D18:0-20:2 | 1.84 | 0.85 | 0.72 | 5.81 | 1.99 | 0.62 | 9.23 | 0.22 | 0.27 | 2.27 | 0.54 | 0.09 |
| 772.5 | D18:0-20:1/P18:1 -22:6 | 2.01 | 0.92 | 0.79 | 6.53 | 1.12 | 0.70 | 24.42 | 1.85 | 0.73 | 4.31 | 0.30 | 0.17 |
| 774.5 | P18: 1-22:5/P18:0-22:6 | 3.62 | 1.01 | 1.42 | 11.68 | 1.93 | 1.25 | 54.54 | 6.01 | 1.62 | 20.35 | 2.54 | 0.82 |
| 776.5 | P18:0-22:5/P18-1 -22-4 | 2.35 | 0.83 | 0.92 | 5.40 | 2.63 | 0.58 | 37 go | 7.64 | 1.12 | 10.62 | 0.93 | 0.43 |
| 778.5 | P18:0-22:4/P18:1 -22:3 | 3.23 | 1.32 | 1.27 | 5.71 | 1.81 | 0.61 | 33.28 | 4.96 | 0.99 | 9.76 | 0.54 | 0.39 |
| 780.5 | P18:0-22:3 | 1.96 | 0.62 | 0.77 | 6.48 | 1.23 | 0.70 | 14.88 | 0.98 | 0.44 | 8.97 | 0.49 | 0.36 |
| 782.5 | P18:0-22:2 | 1.60 | 0.40 | 0.63 | 4.72 | 1.24 | 0.51 | 11.77 | 0.09 | 0.35 | 8.53 | 0.83 | 0.34 |
| 786.5 | D18:2-22:6 | 1.70 | 0.76 | 0.67 | 6.33 | 1.29 | 0.68 | 15.72 | 0.86 | 0.47 | 9.74 | 0.08 | 0.39 |
| 788.5 | D18:1-22:6 | 2.13 | 0.13 | 0.84 | 7.28 | 0.51 | 0.78 | 41.63 | 3.36 | 1.24 | 21.45 | 1.08 | 0.86 |
| 790.5 | D18:1-22:5/D18:0-22:6 | 2.17 | 0.54 | 0.85 | 5.26 | 2.39 | 0.57 | 10.74 | 1.03 | 0.32 | 4.46 | 1.01 | 0.18 |
| 792.5 | D18-1-22-4/D18:0-22:5 | 2.50 | 0.88 | 0.98 | 6.54 | 0.21 | 0.70 | 22.50 | 3.32 | 0.67 | 9.24 | 1.13 | 0.37 |
| 794.5 | D18:1-22:3/D18:0-22:4 | 3.56 | 0.39 | 1.40 | 12.13 | 0.06 | 1.30 | 45.23 | 7.76 | 1.34 | 20.51 | 2.73 | 0.83 |
| 796.5 | D18:0-22:3 | 1.30 | 0.62 | 0.51 | 3.41 | 1.51 | 0.37 | 2.71 | 0.32 | 0.08 | 2.20 | 0.04 | 0.09 |
| Total | 127.17 | 45.11 | 49.95 | 384.24 | 36.33 | 41.25 | 1.394.68 | 99.38 | 41.46 | 700.30 | 59.28 | 28.23 | |
| Plasmalogens | 73.31 | 236.94 | 925.74 | 455.78 | |||||||||
| % Plasmalogens | 57.64 | 61.66 | 66.37 | 65.08 | |||||||||
Lipids were extracted from membranes and analyzed for ethanolamine glycerophospholipid content using multi-dimensional ESI/MS, as described in Experimental Procedures. Results represent the mean +/− SD of three separate experiments. The first number in each pair refers to the number of carbon atoms in the fatty acyl chain. The number after the colon refers to the number of double bonds. The two fatty acyl chain designations are separated by a hyphen. If isobaric molecular species are present in an ion peak, all the major isobaric species are given separated by a back slash. The prefix D indicates a diacyl compound. The prefix P indicates a plasmenyl compound. The prefix A indicates a plasmanyl compound. Results represent the mean +/− SD of three separate experiments.