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. 2002 Jul 10;99(15):10084–10089. doi: 10.1073/pnas.152059399

Figure 2.

Figure 2

Identification of the LMP1-responsive region of the E-cadherin promoter. (A) Schematic representation of human E-cadherin promoter-containing reporter construct and its deletion mutants. The +1 position represents the transcriptional initiation site and restriction enzyme sites used to generate deletion mutants are indicated. (B and C) LMP1 down-regulation of E-cadherin promoter activity in MDCK and MCF-7 cells. The pE-cad constructs were individually cotransfected with pCMV-LMP1 or pCMV2-FLAG. Luciferase activity was normalized by the β-galactosidase activity of pSV2-gal per transfection. Western blotting analysis of the LMP1 protein is shown at the bottom of the figure. The data are an average of at least six independent experiments, and SD is indicated by the vertical bar.