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Polymerized pyrene-actin (4.0 μm) from smooth muscle was titrated with nitromethane (▵). The quenching of pyrene fluorescence was also monitored in the presence of 2.0 μm tropomyosin (•), 1.5 μm caldesmon (▴), and 2.0 μm tropomyosin and 1.5 μm caldesmon simultaneously (○).
