Table 1.
Strains and plasmids used in this study
| Relevant characteristics | Reference/ source | |
|---|---|---|
| Strains | ||
| S6 | seb+ strain | 31 |
| RN4220 | Restriction-deficient mutant of strain 8325-4 | 32 |
| RN4282 | Clinical isolate, contains SaPI1, tst+ | 32 |
| RN6734 | agr+, 8325-4 derivative | |
| RN6938 | RN4282 derivative, SaPI1∷tetM, tst− | 14 |
| RN9131 | RN6734 derivative containing SaPI1 and tst gene | |
| Plasmids | ||
| pRN5543 | pC194 derivative, pUC19 polylinker, Cmr | 12 |
| pRN7034 | Shuttle vector containing promoterless blaZ gene, Emr | Unpublished |
| pRN7040 | pRN7034∷lukS-blaZ transcriptional fusion | This work |
| pRN7041 | pRN7034∷spr-blaZ transcriptional fusion | This work |
| pRN7044 | pRN7034∷tst(a.1)blaZ transcriptional fusion | This work |
| pRN7045 | pRN7034∷tst(a.8)blaZ transcriptional fusion | This work |
| pRN7056 | pRN7034∷tst(a.5)blaZ transcriptional fusion | This work |
| pRN7112 | pRN7034∷seb(b.1)blaZ transcriptional fusion | This work |
| pRN7114 | pRN5543∷seb, intact seb gene | This work |
| pRN7116 | pRN5543∷seb(b.2), truncated seb gene | This work |
| pRN7118 | pRN5543∷tst(a.5), truncated tst gene | This work |
| pRN7119 | pRN5543∷tst(a.8), intact tst gene | This work |
| pRN7123 | pRN5543∷tst(FSh), tst gene with frame-shift mutation | This work |
| pRN7126 | pRN7034∷tst(FSh)blaZ transcriptional fusion | This work |