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. 2002 Jun 28;99(15):10126–10131. doi: 10.1073/pnas.152318699

Figure 1.

Figure 1

Homologous recombination of the Gemin2 locus in mice and characterization of wild-type and Gemin2 targeted preimplantation embryos. (a) An EBFP-Neo-cassette (large green arrows) was inserted into exon 1 of the Gemin2 gene by homologous recombination (dashed lines in red) in mouse embryonic stem cells. Small blue arrows indicate the position of primers used for genotyping and the horizontal blue bar indicates the DNA probe used for Southern blot analysis of EcoRI-digested genomic DNA. Exons are marked in red. (b) Southern blot analysis of Gemin2+/− and wild-type mice with EcoRI-digested genomic DNA. All Gemin2+/− clones identified by PCR showed an EcoRI fragment of 6.5 kb indicative for the mutated allele along with the 9.0-kb wild-type allele fragment. (c) No morphological alterations were observed at the morula (Upper) and blastocyst stages (Lower) in Gemin2−/−, Gemin2+/−, and wild-type embryos. (d) Genotyping by PCR and subsequent Southern blotting showed products corresponding to the wild type (WT, 1.6 kb) and the mutant allele (KO, 1.2 kb).