Table 2.

MAP activity in crude cell extracts of E. coli and B. subtilis

	Specific Activity (U/mg)
Peptide (4 mM)	E. coli (BL5)	B. subtilis wild type (168)	B. subtilis yflG mutant (BFS4611)
MGMM	1.5 ± 0.2	1.5 ± 0.1	1.5 ± 0.1
MAS	1.1 ± 0.2	1.1 ± 0.1	1.1 ± 0.2
MG	0.6 ± 0.1	0.8 ± 0.2	0.4 ± 0.1

Cells in stationary growth phase of each of the strains were collected and sonically disrupted. Supernatants of each of the cell debris were used to measure MAP activity. Totally, three times of measurements were carried out.