Figure 5.
p120 prevents endocytosis of VE-cadherin. The IL-2R-VE-cadcyto chimera was expressed with empty virus (A–C) or in combination with p120 (D–F), p0071 (G–I), or β-catenin (J and K). Cell surface IL-2R-VE-cadcyto was labeled using IL-2R antibody at 4°C, and then cells were transferred to 37°C for 5 min. After acid washing, localization of internalized IL-2R-VE-cadcyto was examined by immunofluorescence microscopy. Expression of the IL-2R-VE-cadcyto polypeptide was verified by using a chicken antibody directed against the myc epitope at the carboxyl terminal tail of the IL-2R-VE-cadherin chimera (B, E, and H). Results from the immunofluorescence experiments are shown quantitatively in L. Bar, 50 μm.