Figure 5.

Subcellular localization of endogenous UBPY. (A and B) HeLa cells were transfected with mock (A) or UBPY siRNA (B) expression vector and stained with anti-UBPY antibody. (C–C″) HeLa cells were transfected with HA-tagged Hrs and double-stained with anti-HA (C) and anti-UBPY (C′) antibodies. (D–D″) HeLa cells were transfected with GFP-tagged SKD1^E235Q and stained with anti-UBPY (D′) antibody. D shows the localization of SKD1^E235Q detected by the GFP fluorescence. C″ and D″ are merged images. Arrowheads indicate colocalization of UBPY with transfected Hrs (C–C″) and SKD1^E235Q (d–d″). N indicates the nucleus. Bar, 20 μm.