Figure 3.
Real-time PCR analysis of expression of kinesins in U. maydis. (A) Graph showing fluorescent intensities relative to PCR cycles, by using complete RNA from the filamentously growing strain AB33, specific primers for all kinesins (Kin1–Kin14) and the housekeeping gene EF1-α. Amplification increases after 20–28 cycles indicating that all genes are expressed. Ct: threshold cycle above background indicating beginning of logarithmic amplification. (B) Comparison of the expression of all kinesins in filamentously growing AB33 and yeast-like control strain AB34 under the same growth conditions demonstrate that kin1 and kin3 are up-regulated in hyphal growth. This corresponds well with the important role of these kinesins in filamentous growth.