Table 1.

Restoration of triad targeting by two-domain α_1S constructs expressed in dysgenic myotubes

Construct	% targeted myotubes*	No. of myotubes analyzed
GFP-α1S (ref. 11)	58	967
GFP-I⋅II	0	361
III⋅IV	0	362
GFP-I⋅II + III⋅IV	73	735
GFP-I⋅II⋅tail	0	799
GFP-I⋅II⋅tail + III⋅IV	28	321
III⋅IV⋅βin	0	400
GFP-I⋅II + III⋅IV⋅βin	2	600

^*Myotubes were classified as “targeted” if any region of the myotubes displayed the characteristic clustered immunolabeling pattern (see Fig. 1) colocalized with RyR1 label, which is indicative of triad labeling. The remainder represents myotubes in which the construct was expressed but not colocalized with RyR1-positive clusters.