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. 2005 Nov;16(11):5215–5226. doi: 10.1091/mbc.E05-06-0504

Figure 2.

Figure 2.

Effect of protease inhibitors and overexpression of proteases on the C-terminal cleavage of PC5A. (A) V5-tagged WT PC5A and the R621A mutant were expressed with or without α1-PDX in HEK293 cells. Western blots were done on conditioned media using the Ab:V5. (B) Ab:V5-Western blot analysis of the conditioned media of CHO-K1 cells, FD11, and FD11-furin (stably overexpressing furin) expressing WT PC5A or its R621A mutant. (C) Ab:V5-Western blot analysis of the media of HEK293 cells transfected with WT PC5A and incubated with different protease inhibitors.