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. 2005 Nov;16(11):5215–5226. doi: 10.1091/mbc.E05-06-0504

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Copyright © 2005, The American Society for Cell Biology

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Figure 4. — Coimmunoprecipitations of endogenous and overexpressed TIMP-2 with either FL-PC5A or PC5A-CRD. (A) HT1080 cells were transfected with either empty pIRES-EGFP + phCMV (Vectors), FL-PC5A + empty phCMV (Control), or FL-PC5A + TIMP-2 or TIMP-2-LP. The media and the cell extracts were immunoprecipitated using Ab:TIMP-2 and resolved by SDS-PAGE and then revealed by Western blot with Ab:V5. (B) Cos-1 cells were transfected with either empty pIRES-EGFP + phCMV (Vectors), FL-PC5A + empty phCMV (Control), or FL-PC5A + TIMP-2. Cell extracts and media were immunoprecipitated with Ab:TIMP-2 or NRS, resolved by SDS-PAGE, and then revealed by Western blot with Ab:V5 or Ab:TIMP-2. (C) HEK293 cells were transfected with PC5A-CRD and TIMP-2. The cell extract and medium were analyzed as in A.