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Displacement of cell surface PC5A by exogenous TIMP-2 and cell surface localization of FL-PACE4 but not PACE4-ΔC. COS-1 cells were transfected with (A and B) FL-PC5A-V5, (C) FL-PACE4-V5, or (D) PACE4-ΔC-V5. Forty-eight hours, later the cells were incubated for 1 h (A) without (Control) or (B) with recombinant human TIMP-2 (20 μM) added exogenously. In all cases cell surface expression of PC5A, PACE4, and PACE4-ΔC were visualized by confocal microscopy with Ab:V5. Bars, 10 μm.
