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. 2005 Nov;16(11):5227–5235. doi: 10.1091/mbc.E05-05-0405

Figure 1.

Figure 1.

Interaction between Mec1 and Rfa1 in a modified two-hybrid assay. (A) Requirement of DDC2 overexpression for the Mec1–Rfa1 interaction. Strain PJ69-4A carrying pBD-MEC1(2-2368) was transformed with pAD-RFA1 and YEpT-ADH1-DDC2 or their control vector. Transformants were streaked on selectable minimum medium containing 10 mM AT. (B) Identification of the Mec1 region required for its Ddc2-dependent interaction with Rfa1. Strain PJ69-4A carrying both pAD-RFA1 and YEpT-ADH1-DDC2 was transformed with pBD-MEC1(2-2368), pBD-MEC1(2-500), pBD-MEC1(2-938), pBD-MEC1(2-1860), pBD-MEC1(2-2140), or pBD-MEC1-85. The kinase domain (filled in dots) and the extreme C-terminal region (black bar) are indicated. Stars denote substitution mutations in mec1-85 (see Figure 2A). Transformants were streaked on a selectable plate containing 10 mM AT. Interaction with Rfa1 was assessed by the growth of transformants.