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Conversion of l-leucine by resting cells of P. freudenreichii TL 34 (A and C) and ITGP23 (B and D) in 60 mM phosphate (pH 6.8) containing 5 mM l-leucine, 10 mM α-KG, 50 μM PLP, and 50 μM TPP at 30°C. Symbols: ▴, leucine; ◊, α-ketoisocaproic acid; ⧫, isovaleric acid. Cell lysis was monitored by determining the decrease in the OD650 (OD) (•) and the amount of proteins released (□).
