FIG. 3.

Effects of pH and NaCl on conversion of [³H] leucine by resting cells of P. freudenreichii TL 34. The cells were incubated for 43 h at 30°C in 60 mM phosphate at three pH values in presence or absence of 2% NaCl. ▤, α-ketoisocaproic acid; ▨, isovaleric acid. The results are expressed in 10³ disintegrations per minute. The results of duplicate experiments are shown and agreed within 14% on average. The following conditions were used: 2 mM l-leucine, 10 mM α-KG, 50 μM PLP, and 50 μM TPP.