TABLE 1.
Catabolism of BCAAs by resting cells of two strains of P. freudenreichii subsp. shermanii incubated for 24 h in 60 mM phosphate (pH 6.8) with different compounds added or omitted
| Strain | Incubation mixturea | Concn (mM)
|
||||
|---|---|---|---|---|---|---|
| Amino acid consumed | α-KG consumed | Glutamic acid | BC α-keto acid | BC acid | ||
| TL 34 | Basal medium | 1.4 | 3.9 | 3.7 | 1.0 | 0.22 |
| Basal medium without Leu | 1.8 | 1.0 | <0.1 | <0.02 | ||
| Basal medium without α-KG | <0.1 | 1.4 | <0.1 | <0.02 | ||
| Basal medium + 10 mM sodium lactate | 1.2 | 3.8 | 4.0 | 0.8 | 0.18 | |
| Control (basal medium without cells) | <0.1 | <0.1 | <0.03 | <0.1 | <0.02 | |
| Ile + α-KG + PLP + TPP | 2.4 | 3.9 | 2.1 | 0.7 | 0.46 | |
| Val + α-KG + PLP + TPP | 1.3 | 4.4 | 3.7 | 0.8 | 0.24 | |
| ITGP23 | Basal medium | 2.4 | 2.3 | 1.4 | 1.4 | 0.42 |
| Basal medium without Leu | 0.5 | 0.4 | <0.1 | <0.02 | ||
| Basal medium without α-KG | 0.4 | 0.8 | <0.1 | <0.02 | ||
| Basal medium + 10 mM sodium lactate | 2.1 | 2.0 | 3.1 | 1.6 | 0.47 | |
| Control (basal medium without cells) | <0.1 | <0.1 | <0.03 | <0.1 | <0.02 | |
| Ile + α-KG + PLP + TPP | 3.8 | 2.7 | 2.3 | 1.5 | 0.44 | |
| Val + α-KG + PLP + TPP | 2.8 | 1.7 | 1.6 | 1.8 | 0.40 | |
a
Basal medium contained Leu, α-KG, PLP, and TPP.