Fig. 5.

Evaluation the influence on the biological behaviors of HGFs in the remodeling phase. (A) Representative images and (B) quantitative analysis of Edu staining of HGFs cultured in MTi, P-MTi, and HP-MTi. Scale bar, 100 μm. (C) Quantitative analysis of the relative proliferation ratio of HGFs cultured in different surfaces. (D) Representative images of the actin cytoskeleton of HGFs cultured on various substrates with phalloidin-FITC (green) staining. Scale bar, 20 μm. (E) Illustration of the Transwell assay scheme. (F) Quantitative analysis and (G) representative images of transwelled HGFs cultured in different surfaces. Scale bar, 200 μm. (H) Representative IF images of FN-1 and COL1A, and (I) representative laser confocal microscopy images of COL3A1 in HGFs. Scale bar, 50 μm. (J) Quantitative analysis of fluorescence intensity of FN-1, COL1A and COL3A1. (K) Levels of FN-1, COL1A and COL3A1 in HGFs. The value is expressed as the means ± SD, with a minimum sample size of 3. (ns, not significant; ∗P < 0.05; ∗∗P < 0.01; ∗∗∗∗P < 0.0001.)