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. 2025 Nov 19;57:514–530. doi: 10.1016/j.bioactmat.2025.11.018

Fig. 6.

Fig. 6

Evaluation the influence on the biological behaviors of HUVECs in the remodeling phase. (A) Representative images and (B) quantitative analysis of Edu staining of HUVECs cultured in MTi, P-MTi, and HP-MTi. Scale bar, 100 μm. (C) Quantitative analysis of the relative proliferation ratio of HUVECs cultured in different surfaces. (D) Representative images of the actin cytoskeleton of HUVECs cultured on various substrates with phalloidin-FITC (green) staining. Scale bar, 20 μm. (E) Illustration of the Transwell assay scheme. (F) Quantitative analysis and (G) representative images of transwelled HUVECs cultured in different surfaces. Scale bar, 200 μm. (H) Representative laser confocal microscopy images of VEGFA in HUVECs. Scale bar, 50 μm. (I) Representative images and (J)quantitative analysis of tube formation assays. Scale bar, 500 μm.Magnify scale bar, 200 μm. (K) Relative mRNA levels of VEGFA and bFGF in HUVECs. (L) Levels of VEGFA and bFGF in HUVECs. The value is expressed as the means ± SD, with a minimum sample size of 3. (ns, not significant; ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; and ∗∗∗∗P < 0.0001.)