Fig. 9.

The regulatory mechanism of H₂ on tissue remodeling. (A) Schematic illustration of H₂ activating the MAPK signaling pathway in HGFs and HUVECs to promote cell proliferation. (B) Western blot image and (C) quantitative analysis of Erk and p-Erk expression in HGFs and HUVECs. (D) Representative IF images and co-location analysis of p-Erk and Dapi in HGFs and HUVECs. Scale bar, 10 μm. (E) Representative laser confocal microscopy images of Ras in HGFs and HUVECs. Scale bar, 20 μm. Quantitative analysis of fluorescence intensity of Ras in (F) HGFs and (G) HUVECs. (H) Schematic illustration of the collection of the supernatants from HGFs for culturing HUVECs. Levels of (I) CXCL8 and VEGFA of the supernatants from HGFs. (J) Representative immunofluorescence images and (K) quantitative analysis of VEGFs in HUVECs cultured by the supernatants from HGFs. Scale bar, 20 μm. (L) Representative images and (M)quantitative analysis of tube formation assays. Scale bar, 200 μm. The value is expressed as the means ± SD, with a minimum sample size of 3. (ns, not significant; ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; and ∗∗∗∗P < 0.0001.)