TABLE 2.
Summary of organosulfur model compound biotransformations by R. erythropolis BKO53a
Compound | Total sulfur in oil (μM)
|
% Remaining | |
---|---|---|---|
Stock | 24 h | ||
DBT | 940 | 0 | 0 |
Benzothiophenes | |||
Benzothiophene | 1,200 | 920 | 77 |
2-MBT | 670 | 230 | 34 |
3-MBT | 970 | 250 | 26 |
5-MBT | 850 | 850 | 100 |
3,5-Dimethylbenzothiophene | 810 | 0 | 0 |
Thiophenes | |||
2,3,5-Trimethylthiophene | 760 | 530 | 100b |
2,5-Di(tert-butyl)thiophene | 250 | 280 | 112 |
3-n-Octylthiophene | 830 | 940 | 113 |
3-Phenylthiophene | 780 | 760 | 97 |
Dialkyl sulfides | |||
Hexyl sulfide | 8,400 | 1,080 | 13 |
Octyl sulfide | 760 | 740 | 97c |
Decyl sulfide | 600 | 690 | 115 |
Dodecyl sulfide | 940 | 940 | 100 |
Assay results are corrected for the presence of the internal standard (dodecyl sulfide).
For 2,3,5-trimethylthiophene, a similar loss was observed for the cell-free control, indicating volatilization.
GC-SCD indicated the appearance of a product peak that was confirmed by GC-MS to be octyl sulfone.