TABLE 3.
Summary of model compound assaysa
Rhodococcus strain | Genotype | μmol removed
|
DBT sp acte (μmol g [dry wt] of cells−1 min−1) | ||
---|---|---|---|---|---|
5-MBTb | Benzothiophenec | Octyl sulfided | |||
JB55 | Δdsz | −0.7 | −0.3 | 1.2 | 0.06 |
JB55(pEBC388) | dszA+B+C+ | 0 | −1.3 | 0.3 | 0.65 |
JB55(pEBC1100) | dszA+B+C+D+ | 1.2 | NTf | NT | 1.14 |
C4-104 | dszA1B+C+ | −0.1 | NT | 8.9g | 0.54 |
C4-224 | dszA2B+C1 | 3.9 | 3.9 | 9.2 | 0.45 |
JB55(pJA103) | dszA+B+C1D+ | 7.5 | 6.9 | 3.5 | 1.02 |
Assays ended at 24 h unless otherwise stated.
The initial amount of 5-MBT was 8.6 μmol.
The initial amount of benzothiophene was 8.6 μmol.
The initial amount of octyl sulfide was 58 μmol.
The initial amount of DBT was 115 μmol; the assay ended at 30 min.
NT, not tested.
The assay ended at 16 h.