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. 2002 Feb;68(2):642–649. doi: 10.1128/AEM.68.2.642-649.2002

FIG. 1.

FIG. 1.

FIG. 1.

FIG. 1.

FIG. 1.

Time courses of relevant parameters of the defined continuous cultures during the experiment with various sulfate and PCE concentrations in the reservoir medium. Culture conditions: stable condition 1 with 1 mM SO42− plus PCE; stable condition 2 with 2.5 mM SO42− plus PCE; stable condition 3 without SO42− and with PCE; stable condition 4 without SO42− and without PCE; pH 7.1 (±0.1); 34.5°C; dilution rate, 0.02 h−1; electron donor, 2.5 mM fructose. PCE was present at 170 (±20) μM (=100%). PCE was omitted from the reservoir medium at day 32 (as indicated by the arrow). Methanobacterium formicicum was added at day 34. (A) Optical densities (○) and sulfide concentrations (▪) in the liquid. (B) Relative cell numbers in the percentages of Desulfitobacterium frappieri TCE1 (○), Desulfovibrio fructosivorans (▪), and Methanobacterium formicicum (•) (for 100%, see total values in Table 2). (C) Concentrations of fructose (▪) and acetate (○) in the culture liquid during sulfate reduction and/or fermentation by Desulfovibrio fructosivorans. (D) Concentrations of PCE (○), TCE (⧫), and cis-DCE (▪) in the culture liquid.