TABLE 6.
Substrate specificity data for Cel5A and Man5
| Polysaccharide (source) | Molecular mass (Da) | Backbone
|
Side chain
|
Sp acta
|
|||
|---|---|---|---|---|---|---|---|
| Residue(s) | Linkage(s) | Residue(s) | Linkage | Cel5A | Man5 | ||
| Mannan (carob) | 3,500 | Mannose | β-1,4 | None | 22 | 72 | |
| Galactomannan (carob) | 40,000 | Mannose | β-1,4 | Galactose | α-1,6 | 71 | 83 |
| Azo-galactomannan (carob) | NAb | Mannose | β-1,4 | Galactose | α-1,6 | 9 | 17 |
| Galactomannan (guar) | 2,000,000 | Mannose | β-1,4 | Galactose | α-1,6 | 69 | 90 |
| Glucomannan (konjac) | 100,000 | Mannose (60%), glucose (40%) | β-1,4 | None | 377 | 97 | |
| β-Glucan (barley) | 250,000 | Glucose | β-1,4; β-1,3 | None | 2,345 | NDc | |
| Carboxymethyl cellulose | 90,000 | Glucose | β-1,4 | None | 616 | ND | |
| Azo-carboxymethyl cellulose | NA | Glucose | β-1,4 | None | 17 | ND | |
| Xyloglucan (tamarind) | NA | Glucose | β-1,4 | Xylose, galactose | α-1,6 | 270 | ND |
| Lichenan (icelandic moss) | NA | Glucose | β-1,4; β-1,3 | None | 76 | ND | |
| Laminarin (L. digitata) | 5,000 | Glucose | β-1,3 | None | ND | ND | |
| Starch (potato) | NA | Glucose | α-1,6 | None | ND | ND | |
| Xylan (birch wood) | NA | Xylose | β-1,4 | None | ND | ND | |
a
For all polysaccharides except azo-galactomannan and azo-carboxymethyl cellulose specific activity is expressed in units (number of reducing sugar equivalents released per minute) per milligram. For azo-galactomannan and azo-carboxymethyl cellulose specific activity is expressed in absorbance units per minute.
b
NA, not available.
c
ND, activity not detected.