FIG. 1.
Expression cassettes for E7 production and export in L. lactis. Schematic structures of native E7 or Nuc-E7 fusions (at left) expressed under control of the lactococcal PnisA promoter and carried by the indicated plasmids. For details of plasmid constructions, see the text and Table 1. Arrows indicate L. lactis promoter sequences of the nisin-inducible promoter (PnisA), solid vertical bars indicate the ribosome binding sites of the usp45 gene, striped bars indicate signal peptides of the usp45 gene; shaded bars indicate E7 coding region, open bars indicate NucB coding sequences, and stems topped with circles indicate transcription terminators (not to scale).