TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Replicon | Characteristic(s)a | Reference or source |
|---|---|---|---|
| Strains | |||
| E. coli TG1 | supE hsd Δ5 thi Δ(lac-proAB) F"(traD36 proAB-lacZΔM15) | 13 | |
| L. lactis MG1363 | Wild type, plasmid free | 11 | |
| L. lactis MG1363 clpP | Eryr, clpP disrupted by single-crossover recombination, plasmid free | 8 | |
| L. lactis MG1363 dnaK | Eryr, dnaK disrupted by single-crossover recombination, plasmid free | 15 | |
| L. lactis NZ9000 | MG1363 (nisRK genes into chromosome), plasmid free | 16 | |
| L. lactis NZ9000 clpP | Strr Rifr Eryr, MG1363 transconjugant carrying nisRK genes and clpP disruption, plasmid free | This work | |
| L. lactis NZ9000 dnaK | Strr Rifr Eryr, MG1363 transconjugant carrying nisRK genes and dnaK disruption, plasmid free | This work | |
| Plasmids | |||
| pBS SK II+ | (ColE1) | Apr | Stratagene |
| pVE8001 | (ColE1) | Apr, PCR fragment encoding trpA transcription terminator | I. Poquetb |
| pBS:NsiI:nuc | (ColE1) | Apr, PCR fragment, with NsiI sites at both extremities, encoding Nuc mature moiety | S. Nouailleb |
| pGEM-T | (ColE1) | Apr | Promega |
| pGEM-E7 | (ColE1) | Apr; PCR fragment encoding E7 | This work |
| pBS-E7 | (ColE1) | Apr; PCR fragment encoding E7 | This work |
| pCYT-Nuc | (pWV01) | Cmr; gene, expressed under PnisA encodes NucB mature moiety | 7 |
| pSEC-Nuc | (pWV01) | Cmr; gene, expressed under PnisA encodes SPUsp-NucB precursor | 7 |
| pCYT-E7 | (pWV01) | Cmr; gene, expressed under PnisA encodes the native E7 protein | This work |
| pSEC-E7 | (pWV01) | Cmr; gene, expressed under PnisA encodes SPUsp-E7 precursor | This work |
| pCYT-Nuc-E7 | (pWV01) | Cmr; gene, expressed under PnisA encodes a NucB-E7 fusion | This work |
| pSEC-Nuc-E7 | (pWV01) | Cmr; gene, expressed under PnisA encodes SPUsp-NucB-E7 precursor | This work |
a
For strains, genotypic and phenotypic characteristics are given; for plasmids, plasmid and cloned-cassette characteristics are given.
b
Unité de Recherches Laitières et Génétique Appliquée, Institut National de la Recherche Agronomique, Domaine de Vilvert, Jouy en Josas, France.