Table 1.

Oligonucleotides used for RT and PCR

Primer			Sequence (5′-3′)
Gene	Typea	Designation
Primers used for RT and RACE-PCR
mcyA	R2	PMR2	CTA GAG TAG TTA CTG GAT CTA
mcyB	R2	mcyBpR2	AAC CCT TGT TGA CGC TCT GT
mcyC	R2	Elke10	CTG CCA AGA GTT CAG CAT CT
mcyD	R2	Topaz4	TTG TTG CCA TTC CTG CAT
mcyE	R2	mcyEpR2	CGT AAA CCT TCA GGG CAT GA
mcyF	R2	mcyFpR2	TGC AGC CTT CCT GAA ATA ATT
mcyG	R2	mcyGpR2	AGC AGT CAT GGA CAG AAT ATT
mcyH	R2	Ebony5	GAC GCT CAA CAT AAC TGG AA
mcyI	R2	mcyIpR2	GTT GCA TCT GAT TGC GAA TTA
mcyJ	R2	mcyJpR2	AAT CCG CTA CAG CCA ACC TT
Primers used for RACE-PCR and RT-PCR
mcyA	R1	Promi5R	GGT AAT CTA TTG AAA CCA GAT G
mcyB	R1	mcyBpR	CGA GTG ACG CTC TAC AAC TT
mcyC	R1	mcyCpR	CCA GTT CTT GAA AGG CTG CT
mcyD	R1a	MpR60R	TTT GTT GAG ACT CGA AAG CTT TTA
mcyD	R1b	MpR15R	GTT TTC GGA TAA GTT CTT TTT ATC
mcyE	R1	mcyER	GAC TTC CAC TAT AGC ATC AAG
mcyF	R1	mcyFR	CTA TCA ATA GAA CCT GTG CGA
mcyG	R1	mcyGR	ACA AGC CCA TAG TGC GGG AAT
mcyH	R1	abcpR	TCT CAG AAA CTT AGA GGC ATT
mcyI	R1	Ebony3	GCC TCA TTA ATT TCC TCC GGT
mcyJ	R1	mcyJpR	TGA TCT CCG GCT TTA AGT TC
Primers used for RT-PCR
mcyB	F	Ruby43	CGG TTA TTT TGC GAC TCC
mcyC	F	Elke12	GAA AAA TTC ATC GTC AAG CT
mcyE	F	mcyDF	GTC TGT CTC TAT AGA TGA ACG
mcyF	F	mcyEF	GAT CTG GCC TTG GAC GGA ATT
mcyG	F	mcyFF	TTG ACC ATC GCA CCT CTA TCA
mcyH	F	abcpF	GAC TTT CCT AAT ATT AGA CGC
mcyI	F	mcyIpF	CTC CCT TAG AAC AAT TTC TCT
mcyJ	F	mcyJpF	GAG CAA CTT TGC TCA AAG ATT
Primers used for RACE ligation and RACE-PCR
		DT89	CGC CAT TTC CAC CTT CTC TTC
		DT88	GAA GAG AAG GTG GAA ATG GCG TTT TGG

^aR, reverse primer (R₁ is upstream of R₂; R_1a and R_1b were used interchangeably, leading to the same results); F, forward primer.