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. 2002 Feb;68(2):449–455. doi: 10.1128/aem.68.2.449-455.2002

Table 1.

Oligonucleotides used for RT and PCR

Primer
Sequence (5′-3′)
Gene Typea Designation
Primers used for RT and RACE-PCR
mcyA R2 PMR2 CTA GAG TAG TTA CTG GAT CTA
mcyB R2 mcyBpR2 AAC CCT TGT TGA CGC TCT GT
mcyC R2 Elke10 CTG CCA AGA GTT CAG CAT CT
mcyD R2 Topaz4 TTG TTG CCA TTC CTG CAT
mcyE R2 mcyEpR2 CGT AAA CCT TCA GGG CAT GA
mcyF R2 mcyFpR2 TGC AGC CTT CCT GAA ATA ATT
mcyG R2 mcyGpR2 AGC AGT CAT GGA CAG AAT ATT
mcyH R2 Ebony5 GAC GCT CAA CAT AAC TGG AA
mcyI R2 mcyIpR2 GTT GCA TCT GAT TGC GAA TTA
mcyJ R2 mcyJpR2 AAT CCG CTA CAG CCA ACC TT
Primers used for RACE-PCR and RT-PCR
mcyA R1 Promi5R GGT AAT CTA TTG AAA CCA GAT G
mcyB R1 mcyBpR CGA GTG ACG CTC TAC AAC TT
mcyC R1 mcyCpR CCA GTT CTT GAA AGG CTG CT
mcyD R1a MpR60R TTT GTT GAG ACT CGA AAG CTT TTA
mcyD R1b MpR15R GTT TTC GGA TAA GTT CTT TTT ATC
mcyE R1 mcyER GAC TTC CAC TAT AGC ATC AAG
mcyF R1 mcyFR CTA TCA ATA GAA CCT GTG CGA
mcyG R1 mcyGR ACA AGC CCA TAG TGC GGG AAT
mcyH R1 abcpR TCT CAG AAA CTT AGA GGC ATT
mcyI R1 Ebony3 GCC TCA TTA ATT TCC TCC GGT
mcyJ R1 mcyJpR TGA TCT CCG GCT TTA AGT TC
Primers used for RT-PCR
mcyB F Ruby43 CGG TTA TTT TGC GAC TCC
mcyC F Elke12 GAA AAA TTC ATC GTC AAG CT
mcyE F mcyDF GTC TGT CTC TAT AGA TGA ACG
mcyF F mcyEF GAT CTG GCC TTG GAC GGA ATT
mcyG F mcyFF TTG ACC ATC GCA CCT CTA TCA
mcyH F abcpF GAC TTT CCT AAT ATT AGA CGC
mcyI F mcyIpF CTC CCT TAG AAC AAT TTC TCT
mcyJ F mcyJpF GAG CAA CTT TGC TCA AAG ATT
Primers used for RACE ligation and RACE-PCR
DT89 CGC CAT TTC CAC CTT CTC TTC
DT88 GAA GAG AAG GTG GAA ATG GCG TTT TGG
a

R, reverse primer (R1 is upstream of R2; R1a and R1b were used interchangeably, leading to the same results); F, forward primer.