Table 2.
Putative intercistronic transcription start points, +1 nucleotides, promoter sequences at −10 and −35, and locations of the transcription start points and the translation start sites (ORF) in the mcyABCDEFGHIJ cluster (1 to 55,000 bp)
| Gene | Location of:
|
Sequence
|
|
|---|---|---|---|
| Transcription start point (bp)a | ORF (bp)a | −35−10+1 | |
| mcyE1 | 24175 | 24173 | ATTTATGTTTAACGGCGTATAAATTTAGAAATTATAGAGAATAGGG |
| mcyE2 | 24187 | 24173 | CACAGCATCCTAATTTATGTTTAACGGCGTATAAATTTAGAAATTA |
| mcyE3 | 24232 | 24173 | GCTTAACTCTCTGGGGTTAAATTTGGGTTTATATTTACTGATAGCA |
| mcyE4 | 24288 | 24173 | AAAATTATTTTTCAAAAGCCATGAGATGTGGTAGGATAGGTAGATG |
| mcyF | 13703 | 13678 | GAGGGGCAGGATATGTTCACAGAAGATTTTAATTTTTAGTCAACAG |
| mcyG | 12883 | 12791 | CCTTGACCATCGCACCTCTATCAGGTACAATTCAAACTTGAGTTGA |
| mcyH | 4692 | 4672 | CTCCTTACTGGTATCCAACTGATCCTAAAGGAAGGACATTTTCAGA |
| mcyI | 3167 | 3017 | CTTATATCAGCAGTTACAAAAAGCACAGACAACTTTTATTAGCGTA |
| mcyJ | 1909 | 1828 | CTGCAAAATCCTTAGGAGGATAATAATTTGGAAAACATCGGCAAAA |
| E. coli σ70 | TTGACA TATAAT | ||
a
Location starting from the 3′ mcyJ end (accession no. AF183408).