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Detection limit of nested PCR assay. (A) Initial PCR products (primers KLJ1/2). (B) Secondary PCR products (primers CPB-DIAGF/R). PCRs were seeded with known quantities of DNA representative of 1 to 10,000 oocysts (as indicated at the top of each lane). Corresponding lanes on gels A and B represent the same seeded sample. The first lanes of gels A and B are molecular weight standards.
