TABLE 1.
Surface water samples that tested positive for Cryptosporidium spp.
| Sample (date)a | Location | Sample identification | MDCb | Molecular results
|
||
|---|---|---|---|---|---|---|
| Nested PCR | NdeI digestc | Sequence resultsd | ||||
| 3/1/99 | Brook SF | SF | NDe | + | − | 3/C. muris |
| 7/12/99 | Quinapoxet River | QR | + | + | − | 3/C. muris |
| 2/1/00 | Stillwater River | SR | ND | + | 1/+ | 1/C. parvum |
| 11/− | 2/C. muris | |||||
| 2/22/00 | Quinapoxet River | + | − | |||
| 3/7/00 | Quinapoxet River | + | ND | |||
| 4/3/00 | Quinapoxet River | + | ND | |||
| 4/4/00 | Gates Brook | GB | ND | + | 11/− | 5/C. parvum |
| 4/4/00 | Brook JF | ND | + | QNSf | QNSf | |
| 10/23/00 | Stillwater River | + | ND | |||
| 11/7/00 | Quinapoxet River | QR1.5, QR2 | − | + | 6/− | 2/C. parvum |
| 12/5/00 | Stillwater River | SR1.5, SR2 | − | + | 10/− | 6/C. baileyi |
| 12/5/00 | Quinapoxet River | + | − | |||
a
That is, month/day/year.
b
Results of MDC samples processed by IFA: +, presumptive positive for C. parvum; −, not found.
c
1/+, One nested PCR clone was cut with NdeI; 11/−, eleven nested PCR clones were not cut with NdeI. For samples 3/1/99 and 7/12/99, the complete nested-PCR products were not cut with NdeI (the nested-PCR clones were not digested individually).
d
3/C. muris, the nucleotide sequences of three nested-PCR clones were most closely related to those of C. muris.
e
ND, not done.
f
QNS, quantity not sufficient.